Journal: International Journal of Molecular Sciences

Article Title: Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice

doi: 10.3390/ijms241210353

Figure Lengend Snippet: The effects of chronic restraint stress on the regulation of serotonin and noradrenaline pathways and transporters in the prefrontal cortex. ( A ) Two-way ANOVA analyses with all groups quantified relative to male controls with Bonferroni multiple comparisons. Male control n = 6, male RS n = 7, female control n = 6, female RS n = 7. ** (On graph) denotes a significant difference between control and RS means: ** = p < 0.01. ** (Under graph) denotes a difference in main effects: ** = p < 0.01. $ Denotes a difference between male and female groups: $$ = p < 0.01. ( B ) Representative immunoblots for TPH2, IDO, MAO-A, SERT, VMAT2, NET and β-actin. TPH2, tryptophan hydroxylase 2; IDO, indoleamine-pyrrole 2,3-dioxygenase; MAO-A, mono-amine oxidase A; SERT, serotonin transporter; VMAT2, vesicular monoamine transporter 2; NET, noradrenaline transporter RS, chronic restraint stress.

Article Snippet: Anti-serotonin transporter (SERT) , Polyclonal Rabbit , ABclonal (Cat# A14171, RRID:AB_2761030) , 1:1000 in TBST.

Techniques: Control, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice

doi: 10.3390/ijms241210353

Figure Lengend Snippet: The effects of chronic restraint stress on the regulation of serotonin and noradrenaline pathways and transporters in the hippocampus. ( A ) Two-way ANOVA analyses with all groups quantified relative to male controls with Bonferroni multiple comparisons. Male control n = 6, male RS n = 7, female control n = 7, female RS n = 6. * (Under graph) denotes a difference in main effects: * = p < 0.05, ** = p < 0.01. $ Denotes a difference between male and female groups: $ = p < 0.05. ( B ) Representative immunoblots for TPH2, IDO, MAO-A, SERT, VMAT2, NET and β-actin. TPH2, tryptophan hydroxylase 2; IDO, indoleamine-pyrrole 2,3-dioxygenase; MAO-A, mono-amine oxidase A; SERT, serotonin transporter; VMAT2, vesicular monoamine transporter 2; NET, noradrenaline transporter RS, chronic restraint stress.

Article Snippet: Anti-serotonin transporter (SERT) , Polyclonal Rabbit , ABclonal (Cat# A14171, RRID:AB_2761030) , 1:1000 in TBST.

Techniques: Control, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice

doi: 10.3390/ijms241210353

Figure Lengend Snippet: Specificity, species, manufacturer, and dilution details of the antibodies used.

Article Snippet: Anti-serotonin transporter (SERT) , Polyclonal Rabbit , ABclonal (Cat# A14171, RRID:AB_2761030) , 1:1000 in TBST.

Techniques: Membrane

Journal: PLoS ONE

Article Title: Serotonin and Dopamine Protect from Hypothermia/Rewarming Damage through the CBS/ H 2 S Pathway

doi: 10.1371/journal.pone.0022568

Figure Lengend Snippet: Cells subjected to hypothermia (black bars) were incubated at 3°C for 24 h, followed by rewarming to 37°C for 3 h, and compared to non-cooled control cells (37°C, gray bars). Cell survival was assessed by adding MTS to the cells upon rewarming and spectrophotometrical formazon measurement. ( A ) DDT-1 cells show natural resistance to hypothermia, which is abrogated by the serotonin transporter (SERT) inhibitor fluoxetine (Fluox, 1 µM, 15 min) and pretreatment with the tryptophan hydroxylase inhibitor parachlorophenylalanine (PCPA, 24 h). ( B to C ) Concentration-dependent inhibition of hypothermic cells death by serotonin (B) and dopamine (C) in SMAC. ( D ) The protective effect of serotonin (30 µM, 15 min) and dopamine (20 µM, 15 min) pretreatment on hypothermic cell death is precluded by inhibition of their respective transporters with fluoxetine (1 µM, 15 min) and vanoxerine (1 µM, 15 min), but unaffected by non-specific receptor antagonists ketanserin (1 µM, 15 min) and spiperone (1 µM, 15 min). ( E ) Serotonin (30 µM, 15 min) and dopamine (20 µM, 15 min) pretreatment prevent caspase3/7 activation induced by hypothermia in SMAC cells, which is precluded by inhibition of their uptake by fluoxetine (1 µM, 15 min) and vanoxerine (1 µM, 15 min). ANOVA tests, different from non-cooled cells (37°C) P<0.05 (*); different from untreated hypothermic cells (Con) P<0.05 (#). Experiments consist of n≥4. Means ± SEM.

Article Snippet: Inhibition of the serotonin transporter SERT (fluoxetine, 1 μM) and dopamine transporter DAT (vanoxerine, 1 μM), however, completely abrogated serotonin and dopamine induced resistance to hypothermic damage in SMAC ( ).

Techniques: Incubation, Control, Concentration Assay, Inhibition, Activation Assay

Journal: PLoS ONE

Article Title: Serotonin and Dopamine Protect from Hypothermia/Rewarming Damage through the CBS/ H 2 S Pathway

doi: 10.1371/journal.pone.0022568

Figure Lengend Snippet: H 2 S content was measured in cell medium of non-cooled cells (gray bars, 37°C) and hypothermic cells (black bars, 3°C) after incubation for 24 h. ( A ) H 2 S content in DDT cells was unaffected by cooling, but reduced by the serotonin transporter (SERT) inhibitor fluoxetine (1 µM, 15 min pretreatment prior to cooling). ( B to C ) Serotonin (30 µM) and dopamine (20 µM) induce H 2 S production in SMAC both at 37°C and 3°C compared o untreated cells (control), which is blocked by inhibition of their respective transporters fluoxetine (1 µM, 15 min) and vanoxerine (1 µM, 15 min). ANOVA tests, different from non-cooled cells (37°C or control) P<0.05 (*); different from untreated hypothermic cells (Con) P<0.05 (#). Experiments consist of n≥4. Means ± SEM.

Article Snippet: Inhibition of the serotonin transporter SERT (fluoxetine, 1 μM) and dopamine transporter DAT (vanoxerine, 1 μM), however, completely abrogated serotonin and dopamine induced resistance to hypothermic damage in SMAC ( ).

Techniques: Incubation, Control, Inhibition